Isolation of Nitrosomonas in pure culture.
نویسندگان
چکیده
Although it has been 68 years since Winogradsky (1890) accomplished the first isolation of nitrifying bacteria, the methods employed have not been improved significantly, and pure cultures remain incredibly difficult to obtain. The literature records numerous attempts to secure Nitrosomonas in pure culture. Nevertheless, the organism has seldom been isolated by dilution techniques. In the relatively few cases where success was claimed, the procedures were tedious and difficult, and the results often uncertain (Frankland and Frankland, 1890; Heubult, 1929; Engle and Skallau, 1937; Bomeke, 1939). The majority of successful isolations have resulted from the plating of enrichment cultures. However, the colonies formed by Nitrosomonas on washed agar or silica gel are not more than 100 ,u in diameter and micromanipulation is required to pick colonies or single cells (Kingma Boltjes, 1935; Meiklejohn, 1950). The most frequently cited explanations for the difficulties encountered in obtaining Nitrosomonas in pure culture by dilution techniques are: (a) the autotrophic nitrifiers develop very slowly; (b) the heterotrophic contaminants in enrichment cultures develop at a rate equal to or greater than that of the nitrifying bacteria; and (c) the solution media employed by Winogradsky, and subsequently used by others for the cultivation of Nitrosomonas, contain insoluble carbonate which adsorbs the cells and prevents dispersed growth. A recent report by Goldberg and Gainey (1955) resolved conflicting views regarding the role of surface phenomena in nitrification by demonstrating that appreciable quantities of particulate matter were not essential for rapid nitrification in solution medium. This suggested that the isolation of Nitrosomonas by dilution techniques should be reconsidered using media as free from
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 76 5 شماره
صفحات -
تاریخ انتشار 1958